The home page allows the user to input details of a variant and request analysis of this by Missense3D-TM. There are two main ways in which to specify the query variant:

1. Please select the button marked "Position on 3D Structure" to input a variant as a residue position on a 3-d structure file. This input page requires knowledge of the position of the residue of interest onto the 3D coordinate file (residue numbering in 3D structures does not always match the residue numbering of the primary amino acid sequence). You can upload your own experimental or model structure (including AlphaFold models).
2. Select the button marked "Position on Protein Sequence" to input a variant as a position within a Uniprot sequence. The Input requires the protein UniProt ID, amino acid position, substitution and the PDB ID of the experimental 3D structure. You cannot upload your own structure here.