Missense3D Portal
Welcome to the Missense3D suite of resources, developed by Professor Michael Sternberg's Group and Dr Alessia David's Group. The Missense3D portal hosts resources developed to characterise and prioritise missense variants (amino acid substitutions) using protein three-dimensional structural information from models or experimental coordinates.


Missense3D predicts the structural changes introduced by an amino acid substitution. It is applicable for the analyse both experimental coordinates and predicted structures.

Missense3D-PPI predicts the structural changes introduced by an amino acid substitution affecting Protein-Protein interfaces. Use this option if your protein structure has multiple chains and the mutation may occur across the interface. It is applicable for the analyse both experimental coordinates and predicted structures.


Missense3D-TM produces predictions regarding the structural impact of missense variants on transmembrane protein structure. Missense3D-TM provides an overall binary prediction as structurally ‘Damaging’ or ‘Neutral’ based on structural features like ‘breakage of a salt bridge’ and ‘cavity altered’.


Missense3D-DB is a database of precomputed structural predictions for ~4M human missense variants.

Covid-19 news

Half of the human population has a defective TMPRSS2 protein that significantly reduces the chance of developing severe COVID-19: read our preprint at https://doi.org/10.1101/2021.03.04.21252931