Missense3D | Structural Bioinformatics Group

Version 2.0 - Updated 22 Dec 2022

Missense3D-PPI predicts the structural changes introduced by an amino acid substitution at a protein-protein interface and is applicable to the analyse both PDB coordinates and homology-predicted structures

Missense3D predicts the structural changes introduced by an amino acid substitution and is applicable to analyse both PDB coordinates and homology-predicted structures.

Choose your input from:

Position on Protein Sequence

The Input requires the protein UniProt ID, amino acid position, substitution and the PDB ID of the experimental 3D structure. A link to the available PDB structures is provided.
You cannot upload your own structure here.

or

Position on 3D Structure

This input page requires knowledge of the position of the residue of interest onto the 3D coordinate file (residue numbering in 3D structures does not always match the residue numbering of the primary amino acid sequence).
You can upload your own experimental or model structure (including AlphaFold models).

Variant information

UniProt ID

PDB code

OR upload here
(5MB limit)

PDB chain ID

Residue Number

Residue ID

Wild-type amino acid (Required just as a check)

Please select a valid amino acid.

Mutant amino acid

Please select a valid amino acid.

Upload your PDB file(s)
(pdb, zip, or tar format)

Please provide variant information. One variant per line.

Example

#PDB #Chain #Residue No. #WildtypeAA #MutantAA
P06280 3hg3 B 52 Cys Arg
P00918 2fos A 107 His Tyr
*Each column is separated by a space.

Example

#PDB #Chain #Residue No. #WildtypeAA #MutantAA
3hg3 B 52 Cys Arg
2fos A 107 His Tyr
*Each column is separated by a space.

Affiliation

For our continued funding, it is important that we track commercial and non-profit use. Please tick the appropriate option. Your job processing will not be affected in any way.

Or choose from examples (click to apply) 1xar (A) | 251 TYR > CYS 1yz3 (A) | 217 LEU > GLN 1z70 (X) | 1348 ALA > PRO 1x3s (A) | 24 LEU > GLN

Medical Disclaimer: Missense3D is intended for purely research purposes. It should not be used for medical or professional advice or any clinical decision making.